dudo klasovity Posted June 11, 2010 Report Share Posted June 11, 2010 Hi! Few weeks ago I put explants and sowed some seeds of rarer sundews on various TC media. Most of the seeds were not very cheap and it is always a dilema for me whether to sow them on reliable but painfully slow regular substrate or take a risk with possibly wrong media or contamination and watch it rot or die.... This time (as usual) my impatience prevailed So I post some pictures of germinating plants and some bigger plants: d.ascendens 'Serra de Ponta Grossa, Campo Largo, Parana,Brasil' d.ascendens 'Itarare,Sao Paulo,Brasil' d.villosa 'Serra do Itipiboca,MG,Brasil' d.neocaledonica d.tomentosa var. glabrata 'Serra do Caraca,MG,Brasil' d.rotundifolia d.anglica on NAA medium d.moorei d.sp.Lantau Island d.broomensis x ordensis d.ordensis 'Lake Argyle,Kimberley,AU' d.roraimae 'Ptari Tepui,Venezuela' d.hirtella var. lutescens 'Cristalina,Goias State,Brasil' Quote Link to comment Share on other sites More sharing options...
Stefan P Posted June 11, 2010 Report Share Posted June 11, 2010 Wow great plants, I must try this (TC) some day Quote Link to comment Share on other sites More sharing options...
natapongw Posted June 12, 2010 Report Share Posted June 12, 2010 Great to see your success, Hope some day I will have collection in vitro like you. My main problem is place to store TC jar, here room temperature is 29-32 C so it is higher than proper condition to keep them Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted June 12, 2010 Author Report Share Posted June 12, 2010 Hi! Thanx for the compliments. My jars are going through hot spell now temps outside reach 33C and since my room is south-oriented, the temps inside are 27-30C under the lights. They have been like that for some time with no visible harm, except for some species slow down the growth a bit. So the temperature is not that big of an issue here. Of cource, these temperatures cannot be used in a long run. Especially seedlings are sensitive to overheating, but far not as much as in regular substrate. Quote Link to comment Share on other sites More sharing options...
jimscott Posted June 12, 2010 Report Share Posted June 12, 2010 Nice work! Quote Link to comment Share on other sites More sharing options...
Binataboy Posted June 13, 2010 Report Share Posted June 13, 2010 Fantastic work, :) Quote Link to comment Share on other sites More sharing options...
UtricSeb Posted June 13, 2010 Report Share Posted June 13, 2010 Great work Dudo, thanks for sharing. Quote Link to comment Share on other sites More sharing options...
cosmo Posted June 18, 2010 Report Share Posted June 18, 2010 Nice cultures, congrats!. How do you manage to clean the seeds and transfer them to media?, I mean they are seeds of less than 2mm, do you transfer them to media(from the flasks containing bleach/water solution)only by using tweezers? Cheers Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted June 18, 2010 Author Report Share Posted June 18, 2010 Hi, cosmo! Tweezers? Oh, no for God's sake! When the mean number of seeds is usually 20-200 seeds per jar....After sterilization and washing with sterilized water in a small beaker, I let them settle to the bottom. The 'unsinkable seeds' (what i like to call them) which stay afloat are usually not viable anyways, so I just use plastic Eppendorf micropipette to suck the settled seeds and water in, then just sguirt the contents of eppendorf in a jar with media and then carefully remove the excess water (again, using eppendorf pipette). Takes about a minute of your life:-) Done Quote Link to comment Share on other sites More sharing options...
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