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Tobias Kulig

TC root cuttings?

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Hi,

Have anyone success with Drosera root cuttings? Protocol for desinfection?

My idea:

1. rinse under tab water with liquid soap.

2. 15min in 10% bleach

3. 3times rinse in steril water

Should i dip in 70% Iso?

Regards

Tobias

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Hi Tobias,

I would add some detergent (0.5% Triton-X) - that breaks the surface tention!

 

You could try to wash them first with water and then sterilze them with two EtOH concentrations (1st: 100%, 2nd: 50%) with detergent and a last "wash" with pure (or maybe better 70%) EtOH (without detergent)... Slightly vortex in between the steps and dry the pieces on a sterile filter paper would, should be worth a try...

 

 

Cheers

Marc

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Should i dip in 70% Iso?

no, i don't think so.

If the root cuttings are not sterilized after 15 mins in 10% bleach, then the contaminants are inside.

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Hi Marc and Bux,

Thanks for informations!!

@Marc: i will try it with your protocoll. It calls simple!

@Bux: you mean 15min in 10% bleach is sufficient? You mean the contaminants are inside the roots? I think surface sterilisation is sufficient! You don't think so?

Best regards

Tobias

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Surface sterilisation might not be sufficient - contamination can also come from inside (also the case for some Nepenthes seeds)... ;-)

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Hi,

Yes, you are right. Its the same like ceph leafs. I have tried some:

1. week good

2. week good

The third and fourth week is ok too, without contamination

In the fifth week i have a nice mushroom culture.....Sterilisation with ppm solution for 6h, 3x rinse for 5min each.

But i don't give up ;0)

Could i try the roots with a similar steri protocol? PPM solution could work good...

Regards

Tobias

Edited by Tobias Kulig

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