loligo1964 Posted August 10, 2011 Report Share Posted August 10, 2011 Every other year or so, I divide up my Cephalotus plants and take a few leaf and rhizome cuttings -- but, more often than not, usually plant them in conventional compost; but here is a tiny piece suspended in 1/2 strength MS media with NAA, now exhibiting new growth. Apologies for the blurred second photo; or as my father once said, "It's a poor worker who blames his tools . . ." Cephalotus follicularis cv. "Hummer's Giant" Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted August 10, 2011 Report Share Posted August 10, 2011 Great Work! :-) Hope it grows fast on that solid solution! Quote Link to comment Share on other sites More sharing options...
loligo1964 Posted August 10, 2011 Author Report Share Posted August 10, 2011 Great Work! :-) Hope it grows fast on that solid solution! Thanks. For whatever reason, I have had better results with solid media than liquid for Cephaotus . . . Quote Link to comment Share on other sites More sharing options...
meizwang Posted August 10, 2011 Report Share Posted August 10, 2011 looks really promising! Are you using PPM in that media? How did you sterilize the explant material? Quote Link to comment Share on other sites More sharing options...
loligo1964 Posted August 10, 2011 Author Report Share Posted August 10, 2011 looks really promising! Are you using PPM in that media? How did you sterilize the explant material? I did use PPM in the media at 1.5 mg/l; and, as a matter of fact, also used a 1:1 solution of PPM to distilled water to sterilize the explant (after rinsing under running water). There was no use of bleach solutions, peroxides, or alcohol at all. This is also the current protocol I now use for all "valuable" seed . . . Quote Link to comment Share on other sites More sharing options...
sativ Posted August 11, 2011 Report Share Posted August 11, 2011 Expect bacterial contamination after switching to PPM- free medium. 1,5ml PPM/l "switch off" bacteria development- they are not killed, just sleeping. Greetings Witek Quote Link to comment Share on other sites More sharing options...
loligo1964 Posted August 11, 2011 Author Report Share Posted August 11, 2011 Expect bacterial contamination after switching to PPM- free medium. 1,5ml PPM/l "switch off" bacteria development- they are not killed, just sleeping. Greetings Witek In my book, there's no PPM-free media until they hit the compost; and I haven't lost a batch yet . . . Quote Link to comment Share on other sites More sharing options...
sativ Posted August 11, 2011 Report Share Posted August 11, 2011 It's also good way ;) Ii got also hidden bacterial contamination developed on medium with PPM, after one year! from introducing this plant (nepenthes) to TC. It's great that You succed, i'm only "this bad uy" who tells You, to be carefull with this culture, and don't passage it in row wit other plants [whay You will have more plants of it] Greetings Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted August 11, 2011 Report Share Posted August 11, 2011 I am using PPM free medium now,,have been for some time actually. It is true that PPM can hide many types of contamination and they do show very quickly when switching to PPM free medium. I use PPM free medium from the very first step of introduction of tissue, to make sure there is no contamination present (if it is it shows within days -funghi or months-bacteria). It is harder to produce a contamination-unaffected tisue in a jar without PPM so I can understand why many people use it. it is up to each TCer to decide what suits him the best. PPM may become an issue when trading and replating the cultures though. Quote Link to comment Share on other sites More sharing options...
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