dudo klasovity Posted March 19, 2010 Report Share Posted March 19, 2010 Hi there!:-) I had a little spare time today so I took some pictures of mostly drosera TC drosera montana var. glabrata drosera ascendens 'red form' nepenthes muluensis germination pinguicula vallisnerifolia drosera admirabilis drosera anglica drosera peltata drosera spatulata Hope u like them!:-) Quote Link to comment Share on other sites More sharing options...
Mantis Posted March 20, 2010 Report Share Posted March 20, 2010 Nice cultures and pics!! Quote Link to comment Share on other sites More sharing options...
Phil Green Posted March 21, 2010 Report Share Posted March 21, 2010 Nice pic's However, are they actually TC ? as in TISSUE culture = CLONES. OR are they seedlings germinated invitro. Out of interest - what do you use to sterilise the seeds ?? Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted March 21, 2010 Author Report Share Posted March 21, 2010 Hi Phil, not all of the depicted plants are coming from seeds. Some come from leaf tissue, therefore i used the expression 'TC' (no need to complicate things since it is not a terminology post) :-) For sterilisation I use regular household bleach, diluted. Thanx for the nice comments. Quote Link to comment Share on other sites More sharing options...
Tha_Reaper Posted March 21, 2010 Report Share Posted March 21, 2010 the household bleach, is that the one with chorine? or the H2O2 bleach? (the one to clean the toilet, or the one to make your hair blonde? ;) Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted March 21, 2010 Author Report Share Posted March 21, 2010 I am natural blond:-) Yes, bleach is the one with chlorine in it. To be more specific, it is a solution that is formed by reaction of chlorine gas with diluted Na lye. Cl2 + NaOH (aq)-----> NaCl + NaOCl +H2O, it is called disproporciation. The reaction is reversible to some degree, so the chlorine itself is the sterilant. I use diluted 0,5%NaOCl (10 times diluted bleach from store. The good thing is that it already contains soap as the surfactant, so it is ready for use:-) Quote Link to comment Share on other sites More sharing options...
MFS Posted March 22, 2010 Report Share Posted March 22, 2010 Hi Dudo, Is this the first time you germinate a tuberous sundew in vitro? I tried with D. auriculata years ago and they germinated and started to grow well, then stalled at about 1cm diameter. Never took it further, I wonder how easy they'd be to deflask. Mine never formed roots or a tuber. On a separate note I just sowed seed of two lowland populations of D. arcturi in vitro. We'll see how they go. The first lot went into 1/3 strength MS with 20g/L sugar, and after having 24h to hydrate, went into the fridge to stratify for 6 weeks. The second lot will go into the same medium with 100ppm GA3, and directly under the grow lights. Have been using 5,000 ppm NaDCC for 6m for disinfestation, followed by three sterile water rinses. Quote Link to comment Share on other sites More sharing options...
Cas Posted March 22, 2010 Report Share Posted March 22, 2010 Very nice - well done! Quote Link to comment Share on other sites More sharing options...
dudo klasovity Posted March 22, 2010 Author Report Share Posted March 22, 2010 Thank you people! MFS: I have done tuberous in vitro before, but only the easy ones so far. D peltata seems to start roots spontanneously and forms a tuber at the tip of it. The tuber formation can be encouraged by greater amounts of sugar in medium or other chemicals(cannot recall the exact name now), depends on the species. Most of the sundews are pretty easy to deflask, even without roots. They root in the regularpeat afterwards. I usually wait for small signs of root formation in vitro prior to deflasking. Good luck with the d.arcturi experiment! I did something similar with d. linearis (normal stratification versus gibberelins), but still no sigh of germination for both batches. Sometimes a great deal of patience pays off:-) Quote Link to comment Share on other sites More sharing options...
MFS Posted March 22, 2010 Report Share Posted March 22, 2010 Well the seed is very fresh, and I have had good germination from seed of D. arcturi on traditional peat:sand before, so fingers crossed! Quote Link to comment Share on other sites More sharing options...
cosmo Posted March 31, 2010 Report Share Posted March 31, 2010 Nice cultures, congratulations. Did you use some kind of growth regulators(auxins, cytokinins?), or just MS media? Regards Quote Link to comment Share on other sites More sharing options...
tuplantacarnivora Posted April 27, 2010 Report Share Posted April 27, 2010 (edited) Hi dudo would you mind sharing your seed sterilisation "protocol"? I mean.. instruments, % of NaClO3, rinses.. any tips Thank you in advance! Edited April 28, 2010 by tuplantacarnivora Quote Link to comment Share on other sites More sharing options...
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