droseradude Posted July 18, 2006 Report Share Posted July 18, 2006 Hello, I'm trying to figure out the proper GA3 concentration for byblis. (not liniflora). So far, I've found "Mix 250mg Gibberellic acid/ 200ml distilled water and let stand for 24 hours. " Besides that, no site has an exact amount of GA3 per amount of water, and searching various cp forums hasn't yielded me anything either. (except in german that is). Does anyone (who has used the stuff successfully) have a certain concentration that they use? This is my first time using GA3 on anything so I don't want to mess up. Thanks in advance, Matt Quote Link to comment Share on other sites More sharing options...
Guest Sheila Posted July 18, 2006 Report Share Posted July 18, 2006 I used about 25mgs to 20mls of water quite successfully last year for Byblis. That didn't stop the seedlings damping off though. 200 mls is a lot to mix up if you are only trying to germinate a few seeds. I bought 100 mg packets of GA3 and divided the contents into 4 equal amounts, it is nearly impossible to weigh out such a small amount. Quote Link to comment Share on other sites More sharing options...
droseradude Posted July 18, 2006 Author Report Share Posted July 18, 2006 Thanks, I think I'll do that. I've never had problems with anything damping off but who knows.. Quote Link to comment Share on other sites More sharing options...
phissionkorps Posted July 19, 2006 Report Share Posted July 19, 2006 Can you post a link to the German site? I will be sowing some Byblis filifolia in the next few days. If you'd like, I can share the important info from the German site with you in English. If you attend or live near a university, go to the bio department and try to find some professors with labs working with GA3. I obtained about 40 microliters of 200 milimole GA3 in ethanol for free, and all you have to do at that point is drop about 20 microliters of that solution into (approximately) a glass of water. Though I have never used that particular solution before, I have used 0.00115 M solution with great success (about 0.004 g in 15mL of water (I think...can't really remember right now)). If you don't have access to a lab, or at least a digital scale and digital pipetter, this could be a problem for you. In that case, you can order your GA3 from here: http://www.super-grow.biz/GA3Powder20.jsp The kit comes w/ a scoop that measures out 0.092g, and a huge table on how much water to use to achieve a certain concentration. I would rather go by molar, but having it worked out in a chart is much easier. 250ppm is a pretty safe bet as to concentration. A little more or little less give or take (depending on the accuracy of your measurements) would be fine, but I would not go above 500ppm no matter what. Quote Link to comment Share on other sites More sharing options...
phissionkorps Posted July 19, 2006 Report Share Posted July 19, 2006 another thing: "Mix 250mg Gibberellic acid/ 200ml distilled water and let stand for 24 hours. " I get: ((0.25g/386g)/0.2L) = 0.000647M That should be a pretty good concentration. As I said, I used 0.00115M, but thats on the strong end of strong, while 0.000575M is on the weaker end of normal, but still pretty much "perfect" for this kind of thing. Go ahead and use the recipe you found, since I'm going to assume you can't get down to the millimole with your measurements. Also though, it depends on the concentration of the GA3 that said recipe was concocted from. The typical concentrations you find for water soluble are about 20-40% (don't quote me on that!), so I'm sure it'll be fine. If it were 90% GA3, there'd definitely be a different recipe involved, but I think most home growers shy away from the alcohol/GA3 mixture, as the amount of GA3 to weigh out (unless you are making a LOT) is in the microgram range. Quote Link to comment Share on other sites More sharing options...
Petiolarissean Posted July 19, 2006 Report Share Posted July 19, 2006 1 gram GA3 to 1liter of water is the perfect mix.The solution should be 1000 PPM strong.Soak seeds for 5 days wet in the refrigerater.The colder the better.Let packets dry,then sprinkle on medium.Remember people!Gigantea grows when it is very cold.Allen has said that when the daytimes temps don't get any warmer than 60F. with night time temps close to 0F. is when they germinate in the millions.Petiolarissean Quote Link to comment Share on other sites More sharing options...
phissionkorps Posted July 19, 2006 Report Share Posted July 19, 2006 1000ppm is too concentrated. Plus, mixing up 1L of solution isn't practical for a few small seeds Quote Link to comment Share on other sites More sharing options...
Petiolarissean Posted July 19, 2006 Report Share Posted July 19, 2006 Ask Lowrie!He'll say the same.That concentration get's the highest germination rate.But hay!If you have a system.And it works.If it ain't broke.Don't fix it.Petiolarissean Quote Link to comment Share on other sites More sharing options...
droseradude Posted July 19, 2006 Author Report Share Posted July 19, 2006 Thanks for the tips. I ordered from the site you posted (last week, should be arriving soon.) It is the 20% water-soluble solution. I hope you two agree on a PPM soon :) Oh yeah, and 1 Liter probably would be a bit too much. I was thinking more around 1 cup. Quote Link to comment Share on other sites More sharing options...
droseradude Posted July 21, 2006 Author Report Share Posted July 21, 2006 Update:: I ended up using 250ML of water (0.25 liters), and mixing in APPROXIMATELY 300mg of 20%GA3. Maybe a bit more, but you can't be exact with that little scoop. Is this good? (I know it's a little more,but its definitely under 350mg of acid). Thanks Quote Link to comment Share on other sites More sharing options...
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