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dudo klasovity

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Posts posted by dudo klasovity

  1. Thanx for nice comments:-)

    @Iggy: The d. moorei is from the seed you have sent to me some time ago. I introduced it in vitro, but wasnt able to force her to form tubers, she just grew and branched. So I deflasked the plants and put them in regular substrate. Cant wait to see the nice yellow flowers! :-) Does this species go dormant for you sometimes? In my conditions it behaves a lot like peltata, keeps on growing kept hot or cool...

  2. Hi!

    It has been some time since I posted pictures here, so I uploaded fresh photos of few plants of mine. February is the shortest month, but the longest and hardest for me, since I wait for the spring so impatiently. :-) Plants like these help me get through it:-) (Some are new species to my collection). I hope you like them:-)

    drosera neocaledonica (taken out of TC 2 days ago)

    DSCF0001-24.jpg

    drosera afra (new species for me and very beautiful plant). Some call it 'the large trinervia',but to me its appearance is miles away from the one of d. trinervia.

    I hope it will flower soon.

    DSCF0002-21.jpg

    DSCF0008-16.jpg

    drosera colombiana 'Curití' young seedlings (I have noticed she likes it rather cold)

    DSCF0005-8.jpg

    a pot of beautiful drosera broomensis x ordensis

    DSCF0022-1.jpg

    drosera faconeri (entering dormancy soon)

    DSCF0025-2.jpg

    drosera hartmeyerorum (taken out of TC 2 days ago), grows very fast, provided hot environment

    DSCF0028-2.jpg

    drosera petiolaris (suprised me how huge it can grow, leaves up to 13cm)

    DSCF0019-1.jpg

    drosera derbyensis

    DSCF0043-1.jpg

    drosera esterhuyseniae x slackii- now, I am not much into hybrids but this one is very colourful and one of the biggest rosetted sundews

    DSCF0037-1.jpg

    DSCF0032-1.jpg

    drosera moorei

    DSCF0073-1.jpg

    drosera x tokaiensis tank

    DSCF0057-3.jpg

    ,,,and roridula gorgonias

    DSCF0015-10.jpg

  3. Thanx for your nice comments:-)

    @Ada: The deflasking is usually the easy part when you stick to the basic rules like to be very thorough with the removal/washing of residual media (otherwise the plant will quickly mold and die), then the humidity needs to be kept very high (in jars it is 100%)-I use 90-100% after deflasking and then bring it down gradually. Another rule is to avoid direct sunlight, overheating and so on. It is not very complicated procedure, but some species insist on giving me a hard time when deflasked;-) Some take several weeks to adjust to normal conditions, other species show no interruption in growth. Most of the drosera species I deflask rootless and they create root system in the substrate and go on with growing. Others demand to have roots generated using hormones in media prior deflasking. It really depends on species and one has to learn how they react to new conditions. There is no book telling you how particular species will react so it is mostly trial-error methodology.(Which in my personal opinion can be turned into much fun;-)

    I am going to introduce some 7 more drosera and pinguicula species in TC tomorrow so I will keep you posted on my experiments:-)

  4. Here I report what has happened in my test jars filled with gelrite-gelled media and some species, apx. 1 month growth on such medium:

    First, here is my control jar of weedy d.capensis. The proliferative growth shows that the composition of the medium should be fine for common drosera:

    DSCF0024-1.jpg

    Drosera burmannii "Humpty Doo"

    DSCF0009-4.jpg

    Drosera sp. Lantau Island . This species I have on regular peat/sand mix as well. It is a very easy species and grows fine, but in vitro it produces enormously large plants, I have never seen on regular substrate:

    DSCF0016-4.jpg

    Drosera neocaledonica started to form hairy roots spontanneously

    DSCF0018-3.jpg

    Drosera ordensis (very hairy form). Grows very fast. I have deflasked about 30 plants and kept this one to see how she will create shoots on 0.7mg/ml BAP

    DSCF0005-2.jpg

    Now this one surprised me. Drosera arcturi. You can see tens of plantlets being formed on one leaf. The reason why this surprised me is that this species in no way froms shoots easily. And this particular plant is on medium WITHOUT hormones.

    DSCF0006-2.jpg

    Drosera affinis- growing slower than i expected, I think it is due to absent roots.I noticed some plants are starting to grow tiny roots on hormoneless medium so soon they will really take off;-)

    DSCF0013-3.jpg

    Species that grows like a salad on this type of media for me is drosera falconeri. I have to replate very often to keep the overcrowded jars managed. Here, 3 experimental jars (no hormones, NAA, BAP):

    DSCF0001-3.jpg

    Here, drosera afra. Very interesting behaviour in TC. Grows really fast, unlike other species, creates roots spontanneously, and when adult, starts to form clumps of plantlets on the base of the root.

    DSCF0036.jpg

    When drosera afra is placed on the medium containing small amount of cytokinin, it encourages her proliferation even more. The plant creates plantlets on the leaves as well. Moreover, she forms plants on the tips of the roots as well. Forming plants from the tips of the roots I have seen only with d. peltata species. Note the opening 'bud' on the tip of the root:

    DSCF0023-3.jpg

    This is my latest achievement that really made my day recently. Although it looks like an ugly piece of shapeless red mass, it is very exciting to watch direct organogenesis from piece of tissue of Drosera ramentacea grow in vitro. I have been trying to do this for some time. Not very pretty, but that is how a successful jar starts. To me beautiful, anyway:-)

    DSCF0030.jpg

    Drosera hartmeyerorum creates extensive root system without induction of auxin

    DSCF0002-1.jpg

    And here are some darlingtonia californica plantlets growing on medium without hormones:

    DSCF0010-4.jpg

    Finally, my first nepenthes species:Nepenthes albomarginata seedlings

    DSCF0025-3.jpg

    ...and Nepenthes benstonei seedlings, growing first tiny pitchers

    DSCF0029.jpg

    Thanx for watching:-)

  5. Hi Sebastian, yes I am using MS medium , but different concentration from other drosera, the best that really works for me is 28%MS (macro and micro) and 25g sucrose, MS vitamins cut to third as well, but inositol and glycine full. For propagation of shoots i dont use hormones, but other chemical at very high level that works better than hormones (proprietary info) and for rooting i use no hormones or 0.5mg/ml auxin (IBA and NAA mostly) for species that are reluctant to root. Hope this helps:-)

  6. Thank you, George and Willy!

    @Sebastian: the petiolaris complex drosera seem actually the most weedy ones under my TC conditions. Never had any problem, they germinate well and then grow very fast until they are mature and I subject them to PGRs and so on. One of the easiest species for me. The only problem I had was with deflasking and acclimatisation of d. ordensis (very hairy clone from Lake Argyle), which was almost impossible to clean of residual nutrients from medium (because of the thick hairs) and often many specimens died of fungal infection when put on peat- but that is another story.

  7. Hello!

    I would like to share some info and pics of my new TC jars. I have had issues with food store grade agar last fall and because several batches shifted the pH of the media several points up, I have lost some cultures. That was the last straw for me and I decided to upgrade my TC work to next level and thwart the drawbacks of using agar.

    Although agar (of high quality) can and does perform very well as a gelling agent, the main issue for me was that the gel was not clear enough to see the first sign of contamination early enough. It is vital for the survival of possibly contaminated culture that unaffected tissues are replated asap. Gelrite forms glassy crystal clear gel where even the smallest contamination can be spotted during initiation and the culture can be saved completely.

    Another good thing about gelrite is that unlike agar (even purest grade), it does not contain any organic compounds (such as phenolics) and therefore it does not stress replated tissues and they grow without interruption. It does contain some salts but i have not observed negative effects. And last thing is that you need only 1/5th of the amount of agar to form a firm gel:-)

    Here are some (agar and gelrite) based cultures:

    d. sp. 'lantau island'

    DSCF00032-1.jpg

    the same but on gelrite

    DSCF0013-2.jpg

    d.natalensis

    DSCF00072.jpg

    a very neglected propagation jar with d. peltata

    DSCF0011-3.jpg

    d. capensis (i use as a control), a shot through the sheer medium

    DSCF0015-2.jpg

    d.californica seedlings

    DSCF0024.jpg

    d.intermedia 'Carolina Giant' seedlings

    DSCF0016-3.jpg

    d.neocaledonica propagation jar

    DSCF0020-2.jpg

    d. neocaledonica rooting jar

    DSCF0022-2.jpg

    d.ordensis propagation jar

    DSCF00292.jpg

    d. ordensis rooting jar (10 days past replating from jar in previous picture)

    DSCF0038.jpg

    DSCF0039.jpg

    d.falconeri

    DSCF0025-2.jpg

    d.hartmeyerorum

    DSCF0035.jpg

    d.afra

    DSCF0042.jpg

  8. Hi, Iggy!

    Phenomenal plants and pictures! How lucky you are to have them in sunny and cold conditions during the winter. "Sunny winter" is an oximoron here where I live.

    I grow mine under artificial lights in a humidity house inside my apartment. It is not optimal, but they grow OK. Outside it is minus 10 to minus 20C often these days. Cant wait for an early spring to put them ouside in the sun:-)

    Good growing! D.

  9. Hey Dani, thanx a lot!

    I have seen your beautiful pics of this species as well. I am glad you backed up my guess. It is indeed very beautiful plant and I thank Iggy for this 'contamination' :-)

    Is it self fertile? I find it interesting that when I manually polinated the flower, it closed immediately (within 5 minutes after pollination). I hope to get some seeds to spread this one around.

  10. Hello,

    I have had this mystery plant in my collection since this summer. I ordered many seeds to introduce some sundews in vitro and I observed that one plant in jar with d. ascendens looked differently. I used no hormones, so it could not have been just morphologically altered ascendens. It must be a different species. I deflasked the plant (single plant from one seed) and I grew it outside in my greenhouse during the summer. It grew fairly fast. It always looked like some form of drosera spatulata to me. Now it put up a flower stalk.A very short hairy one (about 2cm long). I thought to myself, no spatulata has a hairy flower stalk, except from the variety from Gympie. Then I did some searches on the web and foung this Iggys thread

    http://www.cpukforum.com/forum/index.php?s...p;hl=gympiensis

    To me the plant looks exactly the same as mine. I have ordered some seeds (not of this species) from Iggy some time ago,,so could it be a single stray seed?

    Here are some pictures of the plant. I must add, it is the most beautiful spatulata variety (if indeed i am not mistaken about the species).

    What do you think? Thanx for your help.

    DSCF0005-8.jpg

    DSCF0017-5.jpg

    DSCF0016-5.jpg

  11. @Zeeland: d. hilaris I actually received from Adam this summer. Dont know whether they are just two plants that germinated too close (more likely) or a splitted one from roots. As far as I know d. hilaris is not very sensitive to being repotted. I think dormant or non-dormant can be repotted with no troubles, as long as they reach a reasonable size (>1.5cm). Needless to say, that is just my quasi-educated guess:-) Might need to ask someone who had actually done it.

    Thanx for nice comments BTW:)

  12. I would like to share with you some pics I took today. Enjoy:-)

    d. villosa

    DSCF00212.jpg

    d.regia munchong on some insects

    DSCF00022-1.jpg

    d.regia seedlings

    DSCF0017-4.jpg

    d.coccipetala

    DSCF0051-1.jpg

    d.roraimae 'Cerro Adua, Estado Bolivar, Venezuela' and some young d. viridis in the front

    DSCF0049-1.jpg

    d.'esmeraldae' "Cerro Duida, Venezuela'

    DSCF0035-4.jpg

    d.graminifolia

    DSCF0029-3.jpg

    note how much juice thouse thredy leaves are able to produce during digestion

    DSCF0030-3.jpg

    d.camporupestris

    DSCF0023-4.jpg

    d. graomogolensis

    DSCF0018-8.jpg

    d.tomentosa var. glabrata

    DSCF00052.jpg

    d.hilaris

    DSCF00112.jpg

    d.hirtella var. lutescens

    dlutescens.jpg

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