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dudo klasovity

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Posts posted by dudo klasovity

  1. Hi , CP enthusiasts!

    Today as I was checking my plants in the evening I thought about how beautiful this CP genus is and felt like taking some quick random picturesbefore it gets dark. They are mostly of regularly cultivated species, nevertheless I think they look fit at the peak of this season so I would like to share some pictures here on the forum. Hope you like them.

    drosera slackii


    drosera affinis 'Namibia' (normal)


    drosera affinis 'Namibia' (pygmy bush)


    drosera collinsiae


    drosera sp. 'Lantau Island' (Hong-Kong) colony


    drosera adelae group


    ...and some shots of one of the minigreenhouses' top shelf






  2. @Pato:

    Sounds about right.

    Personally, I dont use PPM at all. But it is advisory to use it for beginners to thwart the contamination possibility. And also, I use pH ~5.7. At and lower than 5.5 is more suitable for dionaea than drosera. Good luck with your TCing!:-)

  3. Thanks for the comments

    @Cosmo: D. hartmeyerorum is one of species that dont take long trips in mail very well. I fear Mexico is too distant. And yes, I do work in a lab as a researcher. Not in the plant business though.

    @Pato: for most drosera 20-30%MS with full macro and full vitamins works very well. If you use other gelling agent than agar, like gelrite for example, add 5% more (it picks up and cross-links the Me+, Me2+ cations with -OH ending groups , and thus lowers their concentration a bit).

  4. Hi! After a while, I had some spare time so I took pictures of some of my newly introduced drosera species in TC. Here are some jars, I hope you like them:

    drosera hartmeyerorum (note the extensive root system)


    drosera marchantii ssp. marchantii


    drosera intermedia


    drosera rotundifolia 'Hawks Tor, Bodmin Moor, UK' (freshly replated seedlings)


    drosera hilaris 'Stellenbosch, RSA'



    drosera slackii x capensis


    drosera brevifolia 'Bolsa Nova, Parana, Brasil'


    drosera sessifolia 'Punau, Rio Negro, Brasil'


    drosera trinervia 'Stromsveikloof, RSA'


    drosera ascendens 'Palmeira, Parana, Brasil'


  5. Hi there!:-)

    I took some quick shots of few plants I hope you like them.

    drosera graomogolensis


    pinguicula x Sethos


    drosera spatulata var. gympiensis


    drosera tomentosa var. glabrata


    ...and drosera afra, flowering the first season for me. One of the plants created impresiive triple flower stalk with lots of flowers



    the flowers in detail


  6. Hi, all:-)

    I thought it would be nice to share the outcome of my experiments with supplemented MS medium on some drosera species in vitro. It is well known that the presence of aminoacids in tissue culture media can promote the growth of the plants in a very significant manner (e.g. glycine being part of traditional MS medium). The suplements are sometimes used, such as well known casein hydrolysate and others. The problem with these is that the ratio and amount of aminoacids produced by acidic hydrolysis of caseine is not defined and only aproximate (analysis results may vary considerably). Since I have access to pure L-aminoacids, I did this experiment with various ratios and mixtures of the following: L-Asparagine, L-Tyrosine, L-Arginine, L-Cysteine, L-Glutamine and Glycine. Of course other AA such as Tryptophan, Proline,...can be used as well.

    The important benefit of using AAs in medium is buffering activity due to zwitterionic nature of AAs. Thus, the pH of medium becomes much more stabile during the growth of the plants.

    The amounts of AA exceeded the figures from literature sources in some cases, generally in a ratio of 10-100mg per L. It is known that too high concentration of AA can thwart or stop the development of plants. But given appropiate concentration, the results are obvious, such as in this case of drosera capensis 'Giant' (supplemented medium on the left):


    Some more sensitive species grow on this medium much better than on regular MS.





    d.anglica "Hawaii" started flowering after replating


    d.ascendens 'Palmeira, Parana, Brasil' - the massive germination. The seedlings are less vulnerable to burn by ammonium nitrate. The reduced form of nitrogen (-NH2 group in AA) is more easily available for plants.


    d. glanduligera


    ,,,I have used this medium on tuberous crosses (thankx for the seeds Iggy)


    They were treated with GA3. BTW, according to my HPLC tests, the original 86% GA3 decomposes to 23% purity after autoclaving (20mins, 120C). I dont think it is as much as 99% as sometimes is stated.

    I hope this info will help some TC enthusiasts to enhance the performance or their work:-)

  7. @Dieter: thank you for your insight. I found out yesterday, that the seeds i used for introduction of d.afra in TC I had ordered from you! :-) It was last June and I remember the seeds were just sitting on agar for months doing nothing. I had issues with my agar and medium pH at that time, so maybe that was why. When I replated them to new medium they commenced to germinate. I never tried to sow d. afra seeds on peat (I dont feel comfortable sowing precious seeds on regular substrate), so can you tell me how long it takes for you to germinate them?

    About the tuberous drosera seeds- you have an email :-) I noticed drosera praefolia germinating on gelrite yesterday, which is a good news, will see how she grows. She is a beautiful plant.

    @Cosmo: Thank you. I add the species I dont have to spread the diversity of my collection and species in abundance I swap or sell to finance the experiments. Due to lack of space (I live in an appartment building- no GH).

  8. @Pato: since the plants dont photosynthesize at all or only a little ( carbon in organic form is provided) , you dont need to follow the temperature pattern as that the plants require normally. Therefore the temperature range is very wide. You can stick them in the fridge if you need to slow down the uptake of nutrients. On the other end, temps over 28C are not recommended.

    @Mati: D. hilaris was introduced via sterile seeds.

    @Dieter: It took me about 2 months to grow d. afra to adulthood in vitro. She is a very fast grower, but not on regular substrate. The small plants grow very slowly on peat in my conditions as well. I see you are having the same experience. I havent had the pleasant opportunity to compare/share cultivation info on this species since it is so scarce. I also noticed that she is not a beginners plant and is very prone to fungal attack when sufficient air circulation is absent. I have lost several plants due to this. Another thing is, that she propagates enormously via leaves. Look at this picture of one dead plant. The new plantlets formed literally on every piece of tissue that touched the peat.


    I dont know whether she behaves always like that, or maybe it is caused by 'hormonal memory', since I have used cytokinine in last replating. Has it flowered for you, Dieter?

    ...and yes I have put some tuberous species in vitro, mostly erected species (peltata, moorei, menziesii). This is whata typical culture looks like:


    the good thing about scrambling species is that they dont need to be primed with roots or tubers prior the deflasking. I havent tried rosetted species yet, but I think they might require to be taken out in a form of tubers...also there is much more fuss about the successful germination.

  9. Hi there! I have some new pictures from my minilab I would like to share with you. I hope you like them:-)

    Pinguicula lusitanica setting flowers


    Pinguicula grandiflora seedlings


    Drosera burmannii 'Humpty Doo' with new flower stalk


    Drosera affinis (finally rooting:-)


    Drosera falconeri (it took me lots of killed plants to figure out how to prime this one with roots before deflasking)


    Drosera afra callus culture just replated to produce quazillion plants:-D


    Drosera arcturi 'Gelignite Creek, Tasmania' is for me a tricky plant in TC but I think I have tamed her now she started to propagate nicely:-)


    Drosera hilaris just replated on new tested media I have designed lately. Hope it will be much faster and will work for most species.


    Nepenthes albomarginata


    Nepenthes benstonei


    • Like 1
  10. Hi Sebastian!

    Thanx for correcting the location for me. I guess have I misread your handwriting:-) Thanx for the seeds a lot. I am glad they germinated quite well. When I kept the seedlings warmer than 20C at night they were stagnant in growth. Now I keep them cooler at night together with d. afra and they grow really nicely and are big enough so i commenced to feed them. Thank you for the helpful tips!

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