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dudo klasovity

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Posts posted by dudo klasovity

  1. Hi Naryn,

    learning TCing is pretty much in most cases a self-educating process (unless you can take some university biochemistry/biology tutorial on this subject).

    I recommend reading any book about TC, just type in keywords of what you are looking for on google books or ebay books or other search engine. I do not think you will find a book specialised purely on TC of carnivorous plants, but luckily we have the internet and forums such as:


    or http://www.labflytrap.com/

    or http://www.world-of-carnivores.com/tissue_culture.html

    ..and so on and so forth.......when you do your research you can start experimenting. Getting neccessary skills and experience is possible via experimentation only, but the reading provides many useful information. So far, not too much research have been done in this particular area, so do not expect miracles! ;-) GOOD LUCK!

  2. Thanx for nice comments everybody!

    Peter: You hit the nail exactly on head, in the second paragraph you described what I am trying to avoid by using TC :-) Tedious work with tiny seedlings, endless waiting and slowly bringing the plantlets up, often a Sisyphean task I must say. And you are right, the results didnt come easy, it took some research and losses in the beginning.


    1. Yes, I could have left the plants on medium longer, even for up to 8 months in a fridge if needed. When you mix a good formula it is possible to grow plants at normal temperatures for 2-5 months before deflasking or replating on fresh media.

    2. The recipe I used was worked up by trial-error, altough I started with something resembling diluted MS medium, but since I have access to chemicals, I rather mix my own medium, because it is easier to modify the amount of some components. But often, just 30%MS works just fine. South american species from my experience are quite sensitive to ammonium nitrate though and need better dilution.

    3. No hormones, no GA3 (it is hormone too). For non-dormant seeds you do not need any pretreatment, just as if you sowed them on regular substrate. In fact, when I tried (just out of curiosity) to use hormones (cytokinins and/or auxins), the results were poor. The seedlings often died soon after germination, or were etiolated and unhealthy looking, with feeble growth rate. The seeds contain neccessary hormones in them already, so generally the use of hormones is not recommended.

  3. Thanx for nice comments!

    Dani, this is the one I bought from you not too long ago. It started flowering 2 weeks after I repoted it. Perhaps the plant was already contemplating flower stalk when with you, and for me it was just a lucky pick! haha :yes:

    Binataboy: I treat this one the same way as other south americans, which means strong artificial light, decent temp drop at night and cool days (about 13C at night and 21C day). I do not use the tray method for watering, since I dont think south americans like being waterlogged very much and I water them only from top.

    I think that the substrate is important factor too, for they like an easily draining one. I use my recipe of 20%white peat, 20%life sphagnum moss,20%perlite, 20%fine silica sand and 20% coarse silica sand and river pebbles. This substrate they really love.

  4. Hi there!

    Many times growers have asked the question, how much faster is the propagation of seedlings in vitro than when sowed on a regular substrate. Well, I got my hands on some high quality seeds (thanx Iggy;-)) recently, so I made a little experiment which would give us a hint about the answer to this question (at least as far as some drosera species are involved). For I was very curious about the outcome myself, I picked 3 species for this experiment and compared the germination ratio, survival ratio and also, most importantly, the size and eagerness of the plants to grow.

    Today, 2 months after start, I am capable of submiting a few pictures and can bother you with some figures:-)

    Conditions: - the species I picked were:

    drosera admirabilis

    drosera sp. South Africa (a.k.a drosera sp. Pretty Rosette)

    drosera ascendens 'red form'

    - the plants grew in environmentally more-less identical conditions (humidity, light levels, temperatures,...etc)

    - in vitro cultures contained no hormones, the cultures were not replated

    - experiment took 2 months, the all seeds were sown on substrate/medium the very same day


    drosera admirabilis:

    germination (in vitro): after 12 days, (ex vitro): after 20 days

    germination ratio (in vitro): very high,>95%, (ex vitro): high, apx. 70%

    survival ratio (in vitro): 100%, (ex vitro): 10%

    size (in vitro): almost uniform size of all plants, median of 10mm, (ex vitro): strong variation in size, median of 4mm

    viability (in vitro): >90%, (ex vitro): low, 30%


    drosera admirabilis on peat/sand


    drosera admirabilis, acclimatised seedlings from in vitro:


    drosera sp. 'Pretty Rosette':

    germination (in vitro): after 13 days, (ex vitro): after 13 days

    germination ratio (in vitro): very high,>95%, (ex vitro): very high, apx. 80%

    survival ratio (in vitro): 100%, (ex vitro): 60%

    size (in vitro): uniform size of all plants, diameter 12mm, (ex vitro): almost uniform in size, median of 3mm

    viability (in vitro): >95%, (ex vitro): 80%


    drosera sp.'South Africa' on peat/sand


    drosera sp. 'South Africa' aclimatised from in vitro


    drosera ascendens 'red form'

    germination (in vitro): after 10 days, (ex vitro): after 15 days

    germination ratio (in vitro): very high,>95%, (ex vitro): very high, apx. 70%

    survival ratio (in vitro): 90%, (ex vitro): 80%

    size (in vitro): almost uniform size of all plants, median of 15mm,(ex vitro): almost uniform in size, median of 3mm

    viability (in vitro): >85%, (ex vitro): 50%


    drosera ascendens 'red form' on peat/sand


    drosera ascendens 'red form' from in vitro


    Seeing this, I would say, that the benefit of in vitro propagation of drosera via seeds, is not as much in a provision of high germination rate (it does not vary from the regular ones, when fresh seeds are used), as much as in the fact, that in vitro growth is substantial for high survival rate and rapid growth.

    The final answer, which was, how much faster does the plant grow in vitro than on soil, I am not able to provide. For this, I would need to measure the time the plant reaches maturity (which can be many months). Unfortunatelly, I cannot provide the numbers since my test period was only 2 months, and the data cannot be reached by mere extrapolation of known figures, simply because the growth is not linear, but exponential function of time. Moreover, exponentiality is influenced by many factors, mainly feeding in the early stages.

    Nevertheless, I think the pics give us a pretty good idea about the benefit of TC. I must add, that growing plants this way, also preserves the original DNA spam of the plants since they are not being cloned;-)

  5. Well done Dani! I am not an expert at this, but according to pictures, the hybrid's seed looks just like transient shape between the 2 mother plants! My guess would be that there is a fat chance of you getting the seeds of hybrid plant. Another question is, how viable the seed will be, lets pray for germination now :laugh:

    I attempted to cross d. ascendens x d. tomentosa var. glabrata and the seedpod was full of seeds, but I didnt take the picture. Now I am waiting for potential germination.

    Good luck Dani!

  6. Hi Iggy!

    Beautiful shots and plants! Love your d. alba! The drosera modesta Brookton I had grew "only" 0,6m during the first season after receiving from Australia. How much taller can it get? The place in the groupshot looks like a giant set of greenhouses, do you work in a botanical garden and have spece on your own? Thanx for posting the pics! :roll:

  7. Thanx Peter,

    Yes, that drosera cistiflora comes from TC as fas as I know. This is her 2nd season on regular substrate. Is a newcomer to me, so I am curious about her behaviour during the growing period. Naively hoping for some flowers later on, but have heard that in culture they are reluctant to do so, especially under artificial lights.

  8. Hi I took a few pics today, hope you like them:

    d.cistiflora started to grow recently (the plants look like pitchers- now I mean in baseball terminology) :Laie_97:


    d.camporupestris 'Serra do Cipo, MG, BRA- you think she is trying to tell me with her leaves that she loves me? :roll:




    d."esmeraldae" 'Cerro Duida, VEN'




    h. tatei var. tatei (fast grower, 17cm traps now) beautiful species


  9. Hi Jens!

    Very neat cultures you have:-) I have had troubles transfering d. roraimae into in vitro culture via leaf explants, are yours from seed?

    I see you grow some tuberous species in vitro as well and would like to ask you if they produce corms without hormonal intervention? I have d. auriculata and d. peltata in vitro and they produce tubers easily enough, but have no experience with other species.

    Good luck growing and experimenting! :-)

  10. First of all, thanx for nice comments, I am happy you like the plants.

    Fernando: dont get me wrong, I love the fact that new species are being discovered and described with great intensity at such fast pace, I just wish there was one name for one plant thats all:-)

    MFS: Speaking of witch, an example of d.coccicaulis/venusta/natalensis.......I am not going to dispute about the correct labeling of this species as there are many opinions what this drosera should be called and whether there really is need for more names. Personally, I sense slight difference between members of this complex, so I distinguish between d. coccicaulis and d.venusta/d.natalensis (I take former 2 as synonyms).

    Sean: The plant of drosera peltata var. foliosa is about 25cm tall. The flowers are not on the top of the plant as it continues growing upward. The plant is not branched. It created a massive basal rosette, from each 2-3 erect stems grew later. The plant is green-yellow colour with white flowers. The location is 'Jamieson, Victoria'. Maybe you remember sending me the seeds some time ago (along with other localised peltata varieties depicted above). BTW thank you for them they are my favourite plants! :-)

  11. Thanx for nice comments.

    Fernando: Yes, I meant that one. I wish there was less taxonomy commotion with some species:-)

    Droseraman: You are right. it is regular form. I've corrected the mistake.

    Greg Allan: tuberous : night:8-15C, day: 17-24C, 10hr light, open terrarium

    South American: night:8-15C, day: 17-22C, 15hr light, almost closed terrarium

    South African and tropical: night:15-20C, day:19-25C, 16hrs light, almost closed terrarium, in the summer 23/32C

    lasiocephala: night:20-24C, day: 25C winter, 37Csummer (some go dormant at 25C-falconeri, ordensis,...)

    I use T5 or turbo power save lightbulbs with white fluorescent light and distilled or R/O water.

  12. drosera peltata seedlings


    drosera spatulata (common but beautiful)


    drosera collinsiae flowering


    flower detail


    drosera coccicaulis


    Petiolaris complex....

    drosera petiolaris


    drosera derbyensis


    drosera ordensis, falling into dormancy


    ...and some South American species (some of them newly acquired-thanx Dani:-)

    drosera graomogolensis


    drosera montana var. tomentosa (a.k.a. glabrata or hairless scape)


    drosera ascendens


    drosera ascendens flowers


    drosera graminifolia


    drosera camporupestris


    • Like 1
  13. Hi! We got so much snow today it was hard to walk outside, so i stayed in and took some pics for you. Hope you like them:-)

    drosera platypoda



    drosera erythrorhiza ssp. collina


    drosera menziesii ssp. menziesii (grows like a weed but never flowers for me.....)


    drosera peltata and drosera foliosa


    drosera auriculata


    I have been waiting 18(!) months for these little seedlings of drosera rupicola to sprout!!!

    sometimes patience is rewarded:-)


    Some of my tropical drosera flower this season and sent up unusually long flower stalks, I think it is due to lack of light in the winter.

    drosera madagascariensis



    drosera affinis



    Recently I got some seedlings sprouting, for exaplme a single(!) plantlet of d.filiformis var. filiformis 'florida all red', a beautiful plant when grown up


    ...and some sprouting gemmaeings :-)

    drosera barbigera 'small southern form'


    drosera enodes 'Giant' (not much of a giant yet:-))


    • Like 1
  14. Hi, Carl!

    Yes, I am still feeding the mix to heliamphora (minor and tatei var. tatei). H. minor flowered and produced seeds. There was a short period of discontinuation in feeding her the supplement when her growth became stagnant. So I restored the feeding routine. H. tatei is producing new clump of plantlets rapidly. Soon there will be many plants available:-) As for other plants, I haven't had the time to extend these experiments onto other plants since all my spare time is taken up by tissue culture experiments and finishing my phd. When I have more time and above all, more space, I will get back to this topic with the greatest pleasure! How about your potentially new findings? (Or anyone's for that matter:-))

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