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dudo klasovity

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Posts posted by dudo klasovity

  1. Thanx for nice comments:-)

    Jimscott: Really? For me it is the easiest part, usually I get 90-100% survival rate after deflasking the plants. Dont see any obstacle there. For me the hardest part is to find a suitable medium:-) Some plants are quite picky (not talking only about drosera now).

  2. Hi, I took some pics of some TC jars:-)

    drosera x tokaiensis


    drosera auriculata seedlings


    drosera ascendens germination


    drosera intermedia 'Algonquin park, Ontario, Canada' on semi-liquid medium


    drosera montana


    drosera rotundifolia seedlings


    drosera spatulata 'green'


    drosera burmannii


    part of my setup


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  3. Thank you people!

    MFS: I have done tuberous in vitro before, but only the easy ones so far. D peltata seems to start roots spontanneously and forms a tuber at the tip of it. The tuber formation can be encouraged by greater amounts of sugar in medium or other chemicals(cannot recall the exact name now), depends on the species. Most of the sundews are pretty easy to deflask, even without roots. They root in the regularpeat afterwards. I usually wait for small signs of root formation in vitro prior to deflasking.

    Good luck with the d.arcturi experiment! I did something similar with d. linearis (normal stratification versus gibberelins), but still no sigh of germination for both batches. Sometimes a great deal of patience pays off:-)

  4. I am natural blond:-) Yes, bleach is the one with chlorine in it. To be more specific, it is a solution that is formed by reaction of chlorine gas with diluted Na lye. Cl2 + NaOH (aq)-----> NaCl + NaOCl +H2O, it is called disproporciation. The reaction is reversible to some degree, so the chlorine itself is the sterilant. I use diluted 0,5%NaOCl (10 times diluted bleach from store. The good thing is that it already contains soap as the surfactant, so it is ready for use:-)

  5. Hi Phil, not all of the depicted plants are coming from seeds. Some come from leaf tissue, therefore i used the expression 'TC' (no need to complicate things since it is not a terminology post) :-)

    For sterilisation I use regular household bleach, diluted.

    Thanx for the nice comments.

  6. Hi there!:-)

    I had a little spare time today so I took some pictures of mostly drosera TC

    drosera montana var. glabrata


    drosera ascendens 'red form'


    nepenthes muluensis germination


    pinguicula vallisnerifolia


    drosera admirabilis


    drosera anglica


    drosera peltata


    drosera spatulata


    Hope u like them!:-)

  7. Hi there!

    This sundew is one of my favourites by far and I would like to see her in flower (so far just my wishful thinking;-))). I searched this and other forums and ws for more information about flowering, but with little success. This is my first season with this species.

    My plant started erected growth about 2 weeks ago and it might very well be the fastest-growing sundew I know of. My guestion is, when does it usually flower (reaching what size/age?). I heard it is quite a challenge to make it flower in cultivation. Does she need direct natural sunlight to perform so? Or are artificial lights enough? Is she exigent of feeding, like other fast-growing dews?

    Here I post a pic I of the plant in my terrarium this morning. Thanx for any useful advice! :-)


  8. To get hands on agar is not a problem (at least not here in Czech Republic), you can get it in packets by 10g in any good store with healthy food line and it is very cheap. The problem is, that since agar is not commercially synthesized, but exploited from natural souces, there are no 2 identical packets of agar. Each agar differs in quality. That means that agar works just fine for your plants but one time you buy new packet which is of low quality or contains toxic elements and it might harm your culture (especially the more sensitive ones). That is why it is a good idea to buy pure, TC grade agar. I had really great results with Sigma-Aldrich agar.

    I never used Gelrite nor Gelcarin since SA Agar never disappointed me. Media with agar really are hazy, in comparison with sheer see-through Gelrite media, but for me this is not a disadvantage.

    MFS: You mean PPM? Yes, I use PPM sometimes for really expensive seeds, just to doublecheck on thwarting the contamination, at least in early stages of the growth. I use it incorporated in medium. For disinfection I dont use it. I have heard some people use alcohol, PPM and antibiotic treatment for really stubborn tissues like Nepenthes for example. I mostly work with drosera and pinguicula tissues, and nepenthes seeds which are significantly easier to sterilise.

    So for me it is just bleach and sterilised water, I try to keep it simple if possible.

    For sterilising seeds, I use simply folded filtration paper used in any laboratories. It can withstand the corrosiveness of bleach for prolonged time, so it is really easy to work with and the cheapest thing in the world:-) Tea bags sound too fragile for this , no?

  9. Aflubenov: the pictures of drosera from tissue culture were taken one week after deflasking (deflasking is taking the plants from agar sterile agar medium and planting them in regular substrate, which is NOT sterilised).

    If you wonder what the sundew looked like while in vitro, here are some pics of the same plants on agar:

    drosera admirabilis


    drosera sp. 'South Africa'


    drosera ascendens 'red form' (germinating on medium)


  10. Hi Sebastian!

    Using the seeds for TC has many advantages, amongst which the biggest one is that they are relatively hard to kill during the sterilization sprocess in comparison with e.g. leaf tissue. I use a very simple sterilization protocol, using 1,5% NaOCl solution (30% solution of bleach, which is in fact about 5%NaOCl solution) and one rinse with sterilised water. Thats it. The time period neccessary to sterilize the seeds varies from 2 to 5 minutes (it depends on the species, now I am talking about drosera seeds). Pinguicula seeds need less and nepenthes seeds more time in average.

    Sometimes, when your sterilization is too strong, you bleach the seeds too much, the germination rate is very low and the seedlings dont grow well, or die early. It can also mean, of course, that there is a toxic element contaminating the medium, which can come from low grade chemicals or agar impurities. Most of drosera dont mind low grade agar (used for food), but some do and die. It is a matter of experience to find out details and particularities of propagation of each species.

  11. I left my plants in pots outside this winter and left them there. I could not check on them regularly since I lived abroad, so they were pretty much left to face their fate. Normally they would survive, but this year's winter was/is very harsh with minus 25C, 5 nights in a row and temps similar to that for the rest of the winter. I lost a pot of 6 dionaeas (brown mush) and 2 sarras (dried out completely). Next year I will not leave them unatteneded for such a long time.

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