loligo1964

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Everything posted by loligo1964

  1. I am not sure where a physical copy can be had; but the text of the book; or the doctoral dissertation, in its entirety, can be found at: http://scholarcommons.usf.edu/cgi/viewcontent.cgi?article=4364&context=etd I too was looking for the title some time back . . .
  2. Wistuba handles all of the export and phytosanitary permits on his end and has a broker within the US to ship his orders. No permits are required; and it is included with the shipping . . .
  3. Any Cephalotus can be micropropagated from seed; its vegetative (flat) leaves; the rhizome; and sections of its flower stalks. I have used all of them successfully, for years,. The challenge, as always, is effective sterilization of the explants, without killing the tissue. There were a few threads on Terraforums: http://www.terraforums.com/forums/showthread.php?128901-quot-Eden-Black-quot-F1&highlight=Eden+black Cephalotus follicularis "Eden Black x Self" (seed origin) Cephalotus follicularis "Hummer's Gaint" (flat leaf origin)
  4. Congratulations on, what would have to be, several years of patience . . .
  5. Here are a couple of shots of a particularly stubborn Nepenthes species -- finally beginning to multiply in vitro, after a succession of both media and plant growth regulators -- including 6-BAP and, finally, TDZ (a cotton defoliant of all things): Nepenthes edwardsiana (Tambuyukon) 6 March
  6. Beautiful crosses, as always. 2013 looks to be promising . . .
  7. It is a bit encouraging that the Eden Black-selfed seed have shown such nice color in vitro, especially given the dim lighting usually seen in grow rooms . . . Cephalotus follicularis "Eden Black x Self" 14 February
  8. Usually, the issue is too low a humidity; though seedlings can be adversely affected by high moisture environments. I would also encourage you to increase the photoperiod for your plants, to perhaps fifteen hours . . .
  9. Very beautiful cultures, as always. Congratulations . . .
  10. Here is the last update for 2012 of one of several Cephalotus cultures; and some do appear to look promising. A few of the calli were recently moved into a dilute MS media without any plant growth regulators . . . Cephalotus follicularis "Eden Black x Self" 10 December
  11. A standard 2:1:1 live sphagnum - orchid bark - pumice compost works quite well; and if you're fortunate enough to be dealing with seed, a 2:1 or 1:1 mix of of milled sphagnum or sphagnum peat to horticultural sand is quite successful (and ensures that the slow-growing seedlings are not overgrown by live mosses) . . .
  12. Something to consider about tissue-cultured plants, that could potentially skew some results -- particularly younger plants -- are the high levels of exogenous plant growth regulators often involved in TC . . .
  13. Rapid division is also an artifact of tissue culture processes, where callus tissue -- essentially a "wound" from a cut site, say a flower stalk -- gives rise to new growth . . .
  14. Here is a photo of a root-forming callus (as opposed to those producing shoots or leaves) of the "selfed" seed mentioned earlier. Root formation is often the weakest link in terms of tissue cultured plants; and commercial growers -- to cut costs -- often transfer "plantlets" of many species to compost to root ex vitro. I have found that Cephalotus, in particular, is far more successful and vigorous if there is something more substantial below the soil . . . Cephalotus follicularis "Eden Black x Self" 25 August I was also very encouraged by the bright red coloration of the earliest "vegeta
  15. It still resembles a juvenile N . hamata pitcher to me, since the peristome often doesn't become too pronounced for a couple of years and very in color to a degree. I wouldn't give too much credit to the rumor mill . . .
  16. Alternatively, it can be done fairly cheaply; and there are online sites that describe how to do some sterile technique without the use of a formal laminar flow hood. Also, once the chemicals are purchased, they last a long time in the refrigerator; and tiny amounts are generally used at any given time (100 grams of the gelling agent Gelzan will provide enough for something on the order of 1000-1200 50 ml tubes at 2-4 g/L). The following site offers a club discount to its members and has access to a great deal of materials and techniques. http://www.hometissueculture.org/ DD
  17. I am assuming that you're either in the UK or Europe; so I would do an internet search for tissue culture supplies locally. The TC media is generally a powder that is added in tiny amounts to distilled water, along with sugar, pH adjustment, a gelling agent, occasional plant growth factors (hormones, etc.); and then sterilized. The process can be a bit involved. Here is a good, brief overview of the process and some of what is involved: http://www.flytrapcare.com/tissue-culture-basics.html
  18. Hello, The seedlings can remain in the tray for some time; and I wouldn't be in too much of a hurry, since they have little in terms of a root system at that age. In answer to your question, no, the seedlings weren't germinated in water. That substrate is solid, simply clear; and a tissue culture media, specific for germination . . .
  19. Congratulations on your germination, since I am very fond of seed in the age of TC; and I liked your 50:50 container with both the peat and long fiver sphagnum composts. I have also used both and prefer the peat, if only because it allows for easy transplanting as a peat "plug" without any root damage The only thing that I would be concerned with, down the line, is keeping the seedlings in an overly-humid environment. They are highly susceptible to damp-off fungus (Pythium, for the most part); and the onset can be quite rapid. I have dealt with the issue, for the last couple of years, by addin
  20. Congratulations on the seed-grown plants -- especially lowlanders, whose seed is rarely available while they are still viable . . .
  21. Damn, that's just nasty. What kind of tweaker would steal someone's plant?
  22. They are almost exclusively wasps of the genus,Vespula, commonly referred to as yellowjackets . . .
  23. While trimming away last year’s dried growth from some of my older Darlingtonia plants, I thought that I’d have a little look-see at what had been captured during the 2011 season. The plants seemed to have had enjoyed a particularly favorite meal. Oh, well . . . Darlingtonia californica
  24. Your best bet is to mix the "pourable" powder into a jug of distilled water (about a half teaspoon per gallon in the US) and pour it through your media. What nutrient-uptake Trichoderma assists or contributes to, is inconsequential to the protection afforded from many pathogenic fungi . . .
  25. Here is a shot of a newly-germinated seed, as of this morning . . . Cephalotus follicularis "Eden Black x Self" 11 April 2012