loligo1964

I am not sure where a physical copy can be had; but the text of the book; or the doctoral dissertation, in its entirety, can be found at: http://scholarcommons.usf.edu/cgi/viewcontent.cgi?article=4364&context=etd I too was looking for the title some time back . . .

Wistuba handles all of the export and phytosanitary permits on his end and has a broker within the US to ship his orders. No permits are required; and it is included with the shipping . . .

Any Cephalotus can be micropropagated from seed; its vegetative (flat) leaves; the rhizome; and sections of its flower stalks. I have used all of them successfully, for years,. The challenge, as always, is effective sterilization of the explants, without killing the tissue. There were a few threads on Terraforums: http://www.terraforums.com/forums/showthread.php?128901-quot-Eden-Black-quot-F1&highlight=Eden+black Cephalotus follicularis "Eden Black x Self" (seed origin) Cephalotus follicularis "Hummer's Gaint" (flat leaf origin)

Congratulations on, what would have to be, several years of patience . . .

Here are a couple of shots of a particularly stubborn Nepenthes species -- finally beginning to multiply in vitro, after a succession of both media and plant growth regulators -- including 6-BAP and, finally, TDZ (a cotton defoliant of all things): Nepenthes edwardsiana (Tambuyukon) 6 March

Beautiful crosses, as always. 2013 looks to be promising . . .

It is a bit encouraging that the Eden Black-selfed seed have shown such nice color in vitro, especially given the dim lighting usually seen in grow rooms . . . Cephalotus follicularis "Eden Black x Self" 14 February

Usually, the issue is too low a humidity; though seedlings can be adversely affected by high moisture environments. I would also encourage you to increase the photoperiod for your plants, to perhaps fifteen hours . . .

Very beautiful cultures, as always. Congratulations . . .

Here is the last update for 2012 of one of several Cephalotus cultures; and some do appear to look promising. A few of the calli were recently moved into a dilute MS media without any plant growth regulators . . . Cephalotus follicularis "Eden Black x Self" 10 December

A standard 2:1:1 live sphagnum - orchid bark - pumice compost works quite well; and if you're fortunate enough to be dealing with seed, a 2:1 or 1:1 mix of of milled sphagnum or sphagnum peat to horticultural sand is quite successful (and ensures that the slow-growing seedlings are not overgrown by live mosses) . . .

Something to consider about tissue-cultured plants, that could potentially skew some results -- particularly younger plants -- are the high levels of exogenous plant growth regulators often involved in TC . . .

Rapid division is also an artifact of tissue culture processes, where callus tissue -- essentially a "wound" from a cut site, say a flower stalk -- gives rise to new growth . . .

Here is a photo of a root-forming callus (as opposed to those producing shoots or leaves) of the "selfed" seed mentioned earlier. Root formation is often the weakest link in terms of tissue cultured plants; and commercial growers -- to cut costs -- often transfer "plantlets" of many species to compost to root ex vitro. I have found that Cephalotus, in particular, is far more successful and vigorous if there is something more substantial below the soil . . . Cephalotus follicularis "Eden Black x Self" 25 August I was also very encouraged by the bright red coloration of the earliest "vegetative" leaves on another callus, even under the moderate lighting conditions of a growing room: Here are a couple of other shots of root-producing calli in Muroshige-Skoog-based multiplication media -- both of which currently look like something out of an HP Lovecraft story: 28 August

It still resembles a juvenile N . hamata pitcher to me, since the peristome often doesn't become too pronounced for a couple of years and very in color to a degree. I wouldn't give too much credit to the rumor mill . . .