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Attempt with TC


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#1 Oleksii

 
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Posted 08 May 2012 - 12:54 PM

Hi to all!

I want to try to grow some CP in vitro and my first attempts will be with liquid medium and Utricularia.

And question is: how can I sterilize plants? They are really tiny and I afraid that standard procedure with bleach will kill them.

Do you have any suggestion?



Thanks!

Edited by Oleksii, 08 May 2012 - 12:55 PM.


#2 mantrid

 
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Posted 08 May 2012 - 16:27 PM

The cell wall and cuticle (waxy layer) of the epidermis (outer cell layer) will protect the plant during a quick immersion. I have not done TC so cant give you time plant should be immersed. Probably under a minute or even a few seconds. Im sure someone else will know

#3 mobile

 
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Posted 08 May 2012 - 17:23 PM

Details here: http://thaicps.com/t...tricularia.html

#4 cpa

 
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Posted 09 May 2012 - 15:59 PM

That's a useful link. I quite fancy trying tissue culture myself.

Thanks.

#5 Oleksii

 
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Posted 09 May 2012 - 18:02 PM

Mantrid, I also thought something like that...

Mobile, thanks a lot for a link, it's really useful!

Today I try few methods — from 5 minutes in bleach to just few rinses in bleach and Et-OH. If there is contamination, it become seem after two-three days





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#6 casca

 
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Posted 02 December 2012 - 20:34 PM

I would like to open the link but it's doesn't work for me :(

#7 mobile

 
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Posted 02 December 2012 - 22:04 PM

I would like to open the link but it's doesn't work for me :(

It's not working for me either at the moment. Looks like an issue with their website, as it worked when I initially posted it.

Here's an alternative: http://www.world-of-...tricularia.html

#8 dergeier7

 
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Posted 08 December 2012 - 10:50 AM

I used to disinfect the petiole with 75% acohol then 10% NaOCl but this method often kills explants.Now I find 0.1% mercuric cloride is parctical!

#9 bux

 
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Posted 20 December 2012 - 19:25 PM

mercuric chloride

Should not be used anymore as it is highly toxic!