23 replies to this topic

[sabryberty]

thanks... :) and where can i find the liquid for the tissue culture?

[loligo1964]

sabryberty, on 29 June 2012 - 17:44 PM, said:

thanks... :) and where can i find the liquid for the tissue culture?

I am assuming that you're either in the UK or Europe; so I would do an internet search for tissue culture supplies locally. The TC media is generally a powder that is added in tiny amounts to distilled water, along with sugar, pH adjustment, a gelling agent, occasional plant growth factors (hormones, etc.); and then sterilized. The process can be a bit involved. Here is a good, brief overview of the process and some of what is involved:

http://www.flytrapca...ure-basics.html

[sabryberty]

thank you! : D now I read ... this technique is very effective? I think it is expensive...

[loligo1964]

sabryberty, on 29 June 2012 - 20:37 PM, said:

thank you! : D now I read ... this technique is very effective? I think it is expensive...

Alternatively, it can be done fairly cheaply; and there are online sites that describe how to do some sterile technique without the use of a formal laminar flow hood. Also, once the chemicals are purchased, they last a long time in the refrigerator; and tiny amounts are generally used at any given time (100 grams of the gelling agent Gelzan will provide enough for something on the order of 1000-1200 50 ml tubes at 2-4 g/L). The following site offers a club discount to its members and has access to a great deal of materials and techniques.

http://www.hometissueculture.org/

DD