8 replies to this topic

[loligo1964]

Here are a few shots of Nepenthes in vitro from the last few weeks. The media varies, but is primarily MS-based.

Nepenthes sp. "A"




Nepenthes macrophylla


Nepenthes lowii

Edited by loligo1964, 19 October 2011 - 02:21 AM.

[meizwang]

great to see N. macrophylla invitro.  I wonder if you can coax it to produce multiple shoots in multiplication media after it germinates?

[An D Smith]

Looking good. Have you any info on the sp. A?


Cheers

Andy

[Zagato]

Nice!!!
Where do you found in vitro material???

Thanks

[loligo1964]

Zagato, on 19 October 2011 - 18:52 PM, said:

Nice!!!
Where do you found in vitro material???

Thanks

By in vitro, I am referring to growing the plants -- seeds -- aseptically in tissue culture media; and the materials for TC are now found worldwide . . .

An D Smith, on 19 October 2011 - 12:24 PM, said:

Looking good. Have you any info on the sp. A?


Cheers

Andy

Simply an "unnamed highland Bornean species," for the time being, is what I have been told . . .

Edited by loligo1964, 19 October 2011 - 23:29 PM.

[loligo1964]

Here is a shot from this morning of a culture shown earlier in this thread. They are seed from Nepenthes macrophylla, "flasked" on 9 September; and they currently seem to be enjoying themselves in 1:4 MS with 1 ml/L BAP.

Nepenthes macrophylla -- 14 November

[loligo1964]

Here is an update on one of the highland Nepenthes species illustrated at the beginning of this post; and I am still getting new germination almost six months out. The most successful seeds to date were kept under highland conditions from the very start: relatively warm days (as high as 24˚ C) and cold nights (as low as 10˚ C) . . .

Nepenthes sp. 15 February 2012

[loligo1964]

Here is a brief update of an experimental vial, begun last Fall . . .

Nepenthes "sp. A"  1 December


1 March 2012

[loligo1964]

Here are a couple of shots of a particularly stubborn Nepenthes species -- finally beginning to multiply in vitro, after a succession of both media and plant growth regulators -- including 6-BAP and, finally, TDZ (a cotton defoliant of all things):

Nepenthes edwardsiana (Tambuyukon)  6 March

Edited by loligo1964, 12 March 2013 - 00:22 AM.