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2011 Nepenthes in vitro . . .


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#1 loligo1964

 
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Posted 19 October 2011 - 00:13 AM

Here are a few shots of Nepenthes in vitro from the last few weeks. The media varies, but is primarily MS-based.

Nepenthes sp. "A"
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Nepenthes macrophylla

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Nepenthes lowii

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Edited by loligo1964, 19 October 2011 - 02:21 AM.


#2 meizwang

 
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Posted 19 October 2011 - 02:50 AM

great to see N. macrophylla invitro. I wonder if you can coax it to produce multiple shoots in multiplication media after it germinates?

#3 An D Smith

 
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Posted 19 October 2011 - 12:24 PM

Looking good. Have you any info on the sp. A?


Cheers

Andy

#4 Zagato

 
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Posted 19 October 2011 - 18:52 PM

Nice!!!
Where do you found in vitro material???

Thanks

#5 loligo1964

 
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Posted 19 October 2011 - 23:25 PM

Nice!!!
Where do you found in vitro material???

Thanks


By in vitro, I am referring to growing the plants -- seeds -- aseptically in tissue culture media; and the materials for TC are now found worldwide . . .

Looking good. Have you any info on the sp. A?


Cheers

Andy


Simply an "unnamed highland Bornean species," for the time being, is what I have been told . . .

Edited by loligo1964, 19 October 2011 - 23:29 PM.


#6 loligo1964

 
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Posted 14 November 2011 - 20:44 PM

Here is a shot from this morning of a culture shown earlier in this thread. They are seed from Nepenthes macrophylla, "flasked" on 9 September; and they currently seem to be enjoying themselves in 1:4 MS with 1 ml/L BAP.

Nepenthes macrophylla -- 14 November
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#7 loligo1964

 
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Posted 15 February 2012 - 21:54 PM

Here is an update on one of the highland Nepenthes species illustrated at the beginning of this post; and I am still getting new germination almost six months out. The most successful seeds to date were kept under highland conditions from the very start: relatively warm days (as high as 24˚ C) and cold nights (as low as 10˚ C) . . .

Nepenthes sp. 15 February 2012

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#8 loligo1964

 
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Posted 01 March 2012 - 23:30 PM

Here is a brief update of an experimental vial, begun last Fall . . .

Nepenthes "sp. A" 1 December
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1 March 2012
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#9 loligo1964

 
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Posted 12 March 2013 - 00:19 AM

Here are a couple of shots of a particularly stubborn Nepenthes species -- finally beginning to multiply in vitro, after a succession of both media and plant growth regulators -- including 6-BAP and, finally, TDZ (a cotton defoliant of all things):

Nepenthes edwardsiana (Tambuyukon) 6 March
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Edited by loligo1964, 12 March 2013 - 00:22 AM.

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