Hey Guys,
Thanks for welcoming me to the CPUK Community,
@naoki
For most CP 1/3 MS will work fine for Nepenthes i prefer to use 1/4. In my opinion they grow better with it.
For demonstation purpose i would recommend you to work with some dionaea seeds since they have a thick coat they're easy to sterilize. Working with explants is much more tricky in my opinion and i still have sometimes some trouble with them while sterilizing. Hormones are not always necessary for example most of the drosera species will multiply just fine even without any hormones. When working with nepenthes i prefer to use BAP for multiplication. you can leave them on hormones as long as you wish to. It's always depends on the mass of plants you want to produce. Only thing which is strongly advised to have your plantles for some months on hormone free media before deflasking. Theres no general rule for it, it always depends on the plants and how hormone rich media they were but in general i can say the longer the better.
Matt from Flytrapcare.com ( i think he's also in this forum) wrote an amazing article about tissue culture which helped me a lot when i began. I think you should check it out :-)
https://www.flytrapcare.com/tissue-culture-basics
Best regads
Lukas :-)